plx311 cas9 lentiviral vector (Addgene inc)
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Addgene inc
plx311 cas9 lentiviral vector
Plx311 Cas9 Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector/pLX_311-Cas9+(Plasmid+%2396924)/bio_rxiv__64898__2026__09__24__754062-298-10-13
Average 95 stars, based on 66 article reviews
Plx311 Cas9 Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector/pLX_311-Cas9+(Plasmid+%2396924)/bio_rxiv__64898__2026__09__24__754062-298-10-13
Average 95 stars, based on 66 article reviews
plx311 cas9 lentiviral vector - by Bioz Stars,
2026-10
95/100 stars
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Transduction:Article Title: CD28 co-stimulatory domain enhances efficacy of CER T cell therapy compared to 4-1BB in an ovarian cancer mouse model. Article Snippet: .. ID8 cells were transduced with a firefly luciferase-expressing Luciferase:Article Title: CD28 co-stimulatory domain enhances efficacy of CER T cell therapy compared to 4-1BB in an ovarian cancer mouse model. Article Snippet: .. ID8 cells were transduced with a firefly luciferase-expressing Plasmid Preparation:Article Title: CD28 co-stimulatory domain enhances efficacy of CER T cell therapy compared to 4-1BB in an ovarian cancer mouse model. Article Snippet: .. ID8 cells were transduced with a firefly luciferase-expressing Article Title: Activation of canonical Wnt signaling is required for efficient direct reprogramming into human hepatic progenitor cells Article Snippet: .. The sgRNA oligonucleotides (forward: 5′-CAC CGG AGG CGG CTG CTA CAC CTA-3′ and reverse: 5′-AAA CTA GGT GTA GCA GCC GCC TCC-3′) were annealed, and the annealed sgRNA oligonucleotides were inserted into a Article Title: Serotonergic neuron-glioma interactions drive high-grade glioma pathophysiology Article Snippet: .. To generate a Cas9-expressing cell line, we used a Article Title: Interrogating subclonal heterogeneity of liver cancer with single-cell multi-omics analysis. Article Snippet: .. These sgRNAs were then constructed into a Article Title: Functional genomics using CRISPR-CAS systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof Article Snippet: .. The sgRNA sequences were synthesized as single-stranded oligonucleotides on a CustomArray synthesizer, PCR amplified using Phusion Flash (ThermoFisher Scientific F548L) polymerase (15 cycles), and Gibson cloned into a guide-only Article Title: RHOJ derived peptide promotes chemosensitivity by inhibiting glutamine metabolism in gastric cancer. Article Snippet: .. The following day, the cells were transfected with the desired Article Title: Functional genomics using CRISPR-CAS systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof Article Snippet: .. For each sgRNA, standard desalted short oligonucleotides (Integrated DNA Technologies) were annealed, phosphorylated and cloned into a Article Title: Interrogating subclonal heterogeneity of liver cancer with single-cell multi-omics analysis Article Snippet: Post-infection, the cells were cultured for 15 days in DMEM (HyClone, Cat. SH30243) supplemented with 10% FBS (Sigma, Cat. F0193), 1% L-glutamine (Gibco, Cat. 35050-061), and 1% penicillin–streptomycin (Gibco, Cat. 15140-122). .. These sgRNAs were then constructed into a Construct:Article Title: Interrogating subclonal heterogeneity of liver cancer with single-cell multi-omics analysis. Article Snippet: .. These sgRNAs were then constructed into a Article Title: Interrogating subclonal heterogeneity of liver cancer with single-cell multi-omics analysis Article Snippet: Post-infection, the cells were cultured for 15 days in DMEM (HyClone, Cat. SH30243) supplemented with 10% FBS (Sigma, Cat. F0193), 1% L-glutamine (Gibco, Cat. 35050-061), and 1% penicillin–streptomycin (Gibco, Cat. 15140-122). .. These sgRNAs were then constructed into a Synthesized:Article Title: Functional genomics using CRISPR-CAS systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof Article Snippet: .. The sgRNA sequences were synthesized as single-stranded oligonucleotides on a CustomArray synthesizer, PCR amplified using Phusion Flash (ThermoFisher Scientific F548L) polymerase (15 cycles), and Gibson cloned into a guide-only Polymerase Chain Reaction:Article Title: Functional genomics using CRISPR-CAS systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof Article Snippet: .. The sgRNA sequences were synthesized as single-stranded oligonucleotides on a CustomArray synthesizer, PCR amplified using Phusion Flash (ThermoFisher Scientific F548L) polymerase (15 cycles), and Gibson cloned into a guide-only Amplification:Article Title: Functional genomics using CRISPR-CAS systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof Article Snippet: .. The sgRNA sequences were synthesized as single-stranded oligonucleotides on a CustomArray synthesizer, PCR amplified using Phusion Flash (ThermoFisher Scientific F548L) polymerase (15 cycles), and Gibson cloned into a guide-only Clone Assay:Article Title: Functional genomics using CRISPR-CAS systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof Article Snippet: .. The sgRNA sequences were synthesized as single-stranded oligonucleotides on a CustomArray synthesizer, PCR amplified using Phusion Flash (ThermoFisher Scientific F548L) polymerase (15 cycles), and Gibson cloned into a guide-only Article Title: Functional genomics using CRISPR-CAS systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof Article Snippet: .. For each sgRNA, standard desalted short oligonucleotides (Integrated DNA Technologies) were annealed, phosphorylated and cloned into a Transfection:Article Title: RHOJ derived peptide promotes chemosensitivity by inhibiting glutamine metabolism in gastric cancer. Article Snippet: .. The following day, the cells were transfected with the desired |